Our Faculty

Jason Kuehner

Assistant Professor of Biology


Contact Information

617-975-9698


Office Hours

Office: Wilkens Science Center, Room 209-B

Office hours: Monday and Wednesday, 3:00 p.m.- 4:00 p.m.; Tuesday and Thurday, 11:00 a.m.- 12:00 p.m., by appointment also

Education

Ph.D., University of Wisconsin-Madison; B.A., Cornell College



What I Love About Emmanuel:

I love the breadth and depth of learning that is ingrained within the liberal arts and sciences curriculum. I love the close interaction between faculty and students in the classroom and research laboratory. I love being part of a dynamic campus community that embraces its rich history and surrounding Boston resources.

Courses I Teach

  • BIOL 1105 - Introduction to Cellular and Molecular Biology
  • BIOL 1106 - Introduction to Organismic and Evolutionary Biology
  • BIOL 2123 - Genetics
  • BIOL 2301 - Experimental Biology

Publications + Presentations

Peer-Reviewed Scientific Publications:

  • Graber, J.H., Nazeer, F.I., Yeh, P., Kuehner, J.N., Borikar, S., Hoskinson, D.C., and Moore, C.L. (2013). DNA damage induces targeted, genome-wide variation of poly(A) sites in budding yeast. Genome Research. 23(10): 1690-1703.
  • Gordon, J.M., Shikov, S., Kuehner, J.N., Liriano, M., Lee, E., Stafford, W., Poulsen, M.B., Harrison, C., Moore, C., and Bohm, A. (2011). Reconstitution of CF IA from overexpressed subunits reveals stoichiometry and provides insights into molecular topology. Biochemistry. 50(47): 10203-10214.
  • Kuehner, J.N., Pearson, E.L., and Moore, C. (2011). Unravelling the means to an end: RNA Polymerase II transcription termination. Nat. Rev. Mol. Cell Biol. 12(5):283-294.
  • Cloud-Hansen, K.A.*, Kuehner, J.N.*, Tong L., Miller, S., and Handelsman, J. (2008). Money, sex, and drugs: a case study to teach the genetics of antibiotic resistance. CBE Life Sci. Educ. 7(3): 302-309. *authors contributed equally to this work.
  • Kuehner, J.N. and Brow, D.A. (2008). Regulation of a eukaryotic gene by GTP-dependent start site selection and transcription attenuation. Mol. Cell. 31(2): 201-211. [Selected as cover article]
  • Steinmetz, E.J., Warren C.L., Kuehner, J.N., Panbehi, B., Ansari, A.Z., and Brow, D.A. (2006). Genome-wide distribution of yeast RNA polymerase II and its control by Sen1 helicase. Mol. Cell. 24(5): 735-746. [Selected as cover article]
  • Kuehner, J.N. and Brow, D.A. (2006). Quantitative analysis of in vivo initiator selection by yeast RNA polymerase II supports a scanning model. J. Biol. Chem. 281(20): 14119-14128

Research Presentations: (underlined students mentored by J. Kuehner)

  • Brioso, S.M.T., Moore, C.L., and Kuehner, J.N. (2015). Investigating the role of RNA 3'-end processing factors in the yeast cell wall integrity pathway. Eastern New England Biological Conference, Boston, MA. (Poster presentation)
  • Kelly, K.E., Tallo, T.W., Moore, C.L., and Kuehner, J.N. (2015). The secret life of RNA processing factors: Investigating their role in repairing UV-damaged DNA. Eastern New England Biological Conference, Boston, MA. (Poster presentation)
  • Kuehner, J.N., Duffy, H., and Moore, C.L. (2014). Stimulation of RNA Polymerase II ubiquitination by yeast RNA 3' processing factors is a conserved DNA damage response in eukaryotes. Yeast Genetics and Molecular Biology Conference, Seattle, WA. (Poster presentation)
  • Kuehner, J.N., Nazeer, F.I., and Moore, C.L. (2011). Coupling of RNA 3'-end processing with the DNA damage response. Canadian RiboClub Conference. Orford, Quebec. (Platform talk and poster presentation)
  • Kuehner, J.N., and Moore, C.L. (2010). Mutations in the yeast nuclear mRNA cap-binding protein Cbp80 suppress a Pcf11 mutant defective for binding RNA Polymerase II. Gordon Conference: Post-transcriptional Gene Regulation. Newport, RI. (Poster presentation)
  • Kuehner, J.N. (2009). EMBO Conference: Messenger RNA 3'-ends and Gene Expression. Oxford, England. (Participant)
  • Kuehner, J.N. and Brow, D.A. (2008). Regulation of a eukaryotic gene by GTP-dependent start site selection and transcription attenuation. EMBO Conference: Gene Transcription in Yeast. Sant Feliu de Guizols, Spain. (Platform talk)
  • Kuehner, J.N. and Brow, D.A. (2007). Sen1-dependent attenuation of yeast RNA polymerase II transcription. RNA Society Conference, Madison, WI. (Platform talk)
  • Kuehner, J.N., Steinmetz, E.J., and Brow, D.A. (2007). Sen1-dependent attenuation of yeast RNA polymerase II transcription. Cold Spring Harbor Conference: Mechanisms of Eukaryotic Transcription. Cold Spring Harbor, NY. (Poster presentation)
  • Kuehner, J.N. and Brow, D.A. Quantitative analysis of in vivo initiator selection by yeast RNA polymerase II supports a scanning model. Yeast Genetics and Molecular Biology Conference, Princeton, NJ. (2006). (Poster Presentation)

Peer-Reviewed Teaching Publications:

  • Cloud-Hansen, K.A.*, Kuehner, J.N.*, Tong L., Miller, S., and Handelsman, J. (2008). Money, sex, and drugs: a case study to teach the genetics of antibiotic resistance. CBE Life Sci. Educ. 7(3): 302-309. *authors contributed equally to this work.

Grants + Recognition

Funding Support:

  • National Science Foundation Research Opportunity Award, 2015 ($37,432)
  • Emmanuel College Faculty-Student Research Grants (2012-2015)
  • NIH/NIGMS K12 Postdoctoral Fellowship (IRACDA TEACRS Program; Tufts University)
  • Tri-Beta Biological Honor Society Undergraduate Research Grant

Awards/Recognition:

  • HHMI Teaching Fellow in Classroom Education and Mentoring (University of Wisconsin-Madison)
  • Biochemistry and Molecular Biology Outstanding Senior Award (Cornell College)
  • Phi Beta Kappa Honor Society (Cornell College)

Research Focus

Cells are frequently subjected to stressful conditions, both upon exposure to byproducts of normal metabolism (e.g. reactive oxygen species) and environmental agents (e.g. ultraviolet light). The ability of cells to cope with stress may be a key factor that determines life span, and failure to effectively react to stress can contribute to chronic diseases such as cancer. To counteract these dangers, cells employ a variety of stress responses that coordinate changes in gene expression with recognition and repair of stress-induced damage. Reprogramming of gene expression has largely been studied at the level of transcription (DNA-->RNA) and translation (RNA-->Protein). However, recent evidence indicates that RNA 3'-end processing provides an important yet under-appreciated means through which cells respond to stress. My laboratory is investigating connections between RNA processing factors and two different cell stress pathways, including the DNA damage and cell wall damage response. Using a combination of molecular biology, genetics, biochemistry, and bioinformatics, we aim to uncover novel stress response mechanisms in the yeast model system that may be shared across species.

Supervised Research Students at Emmanuel College

Student (class year)

Term Current/last known position
Christine Tuohy ('16) Jan. 2015 - present Emmanuel undergraduate
Samisa Brioso ('15) June 2014 - present Emmanuel undergraduate
Kaitlyn Kelly ('15) Jan. 2014 - present MGH Master of Science in Nursing Program
Thomas Tallo ('16) Jan. 2014 - present Emmanuel undergraduate
Hilary Duffy ('14) June 2013 - Aug. 2013 Admin. Assistant, Northeastern University